Four enzyme cleaners are necessary for superior cleaning outcomes.

Lipase enzymes break down fat to cleave fatty acid residue from the glycerol residue into a neutral fat or a phospholipid, Amylase enzymes break down starch to catalyze the hydrolysis of starch to sugar to produce carbohydrate derivatives, Carbohydrase enzymes break down starches to a lower level to catalyze the hydrolysis of higher carbohydrates to lower forms, and Protease enzymes break down proteinases and peptidases, including blood, to catalyze the hydrolytic break down of proteins.

The four enzyme cleaners break down bioburden that is commonly found on contaminated surgical instruments;
lipase enzymes for fats,
amylase enzymes for starches,
carbohydrase enzymes for high level starches, and
protease enzymes for proteinaceous debris (blood).

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Enzyme Cleaners Optimal Cleaning Temperatures

The optimal temperature for maximum enzymatic cleaning performance peaks at 137 degrees Fahrenheit (137 degree Fahrenheit = 58.33 degree Celsius). The cleaning activity of the enzymes at temperatures below and above this point is less but does offer cleaning value. The cleaning activity of the enzymes does not stop at this temperature, but is does lessen as the temperature increases.

Enzyme Cleaners Optimal Dosage Rates

Some manufacturers suggest that a significantly smaller dosage of their enzymes are needed. A key factor affecting the efficacy of any enzyme is the concentration level of the enzyme as it is packaged by the manufacturer prior to dilution. It is important that the enzymatic cleaning product deliver the four enzymes that are necessary for removing the types of bioburden commonly found on contaminated surgical instruments.

Claims that an enzymatic "multi" is irrelevant. What is relevant is stating which enzymes, of the four essential enzymes, are included in the product formulation. 

The optimal application time for maximum surgical instrument cleaning detergent performance depends of the level of soil hydration, encrustation, the amount of soil, and the mass of items being cleaned. Claims as to enzymatic products cleaning within minutes are fallacious without specifics as to the above parameters.

It is commonly stated that enzymes clean. Enzymes alone do not clean effectively. The primary function of enzymes is to break down soil, usually proteinaceous bioburden. For a product to clean effectively, surface cleaning detergents are necessary, that will remove the soil from the surface, while enzymes break down the bioburden.

Four types of enzyme cleaners with surface cleaning surgical instrument detergents are necessary to achieve the highest quality cleaning outcomes.

surgical instrument detergents
Enzymes with detergents are necessary.
Four types of enzymes break down bioburden while the surface cleaning detergents remove the soil from the surface, for residue free medical devices.